Composite

Part:BBa_K400000:Design

Designed by: Jonghwa Kim   Group: iGEM10_Korea_U_Seoul   (2010-10-21)


composite heavy metal-detector(Zn, Cd, As)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2105
    Illegal PstI site found at 5490
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2105
    Illegal PstI site found at 5490
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2105
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2105
    Illegal PstI site found at 5490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2105
    Illegal PstI site found at 5490
    Illegal NgoMIV site found at 2694
    Illegal NgoMIV site found at 3731
    Illegal AgeI site found at 4516
    Illegal AgeI site found at 4628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1144
    Illegal BsaI.rc site found at 5410


Design Notes

Each of the parts were cleaved with Endonuclease and ligated with pSB1C3 vector. First, a promoter and a reporter gene were amplified by PCR and two of the pairs were ligated with pSB1C3 vector. Second, another promoter and reporter gene were put together by PCR and then ligated with pSB1C3 vector with the other two-pair-fragment. Three pairs were finally put in a vector and it underwent point mutation during the procedure because it had multiple cleavage sites.


Source

a soil bacterium

References